Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:76105 Suzuki M, et al., Genetic modifier loci affecting survival and cardiac function in murine dilated cardiomyopathy. Circulation. 2002 Apr 16;105(15):1824-9
• ID
  MGI:2389059

Genes

Gene	Assay Type
Hrtfm1	visible phenotype
D2Mit327
D2Mit325
D2Mit418
Ttn	reported elsewhere

Notes

• Experiment
  100 microsatellite markers at an average spacing of 15 cM were screened in 70 (C57BL/6J x DBA/2J-Tg(Myhca-Casq2)1Mord)F1 x C57BL/6J backcross animals and 50 (C57BL/6J x DBA/2J-Tg(Myhca-Casq2)1Mord)F1 x DBA/2J backcross animals to identify QTLs associatedwith cardiac function and survival. (C57BL/6J x DBA/2J-Tg(Myhca-Casq2)1Mord)F1 hybrid mice show markedly decreased survival and heart function compared to parental DBA/2J-Tg(Myhca-Casq2)1Mord mice.
  
  In the first backcross, a QTL linked to heart failure,Hrtfm1, mapped to mouse chromosome 2 at 40.4 cM with a peak LOD=7.8 at D2Mit327. The QTL range of Hrtfm1 spans 38.3 cM - 43.5 cM. Haplotype analysis further refined interval to a 1.1 cM region between D2Mit325 and D2Mit418. DBA/2J-derived alleles confer increased survival at Hrtfm1 with a dominant mode of inheritance. A possible candidate gene for Hrtfm1 is titin (Ttn).
  
  In the second backcross, a QTL linked to both cardiac function and survival, Hrtfm2, mapped to 76.2 cM on mouse Chromosome 3 with a peak LOD=9.3 at D3Mit86. The QTL range of Hrtfm2 spans 71.8 cM - 72.9 cM. Haplotype analysis further refined the interval to a 5.2 cM region between D3Mit320 and D3Mit260. C57BL/6J-derived alleles confer decreased heart function and survival at Hrtfm2 with adominant mode of inheritance.