Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  4
• Reference
  J:77010 Mendoza-Lujambio I, et al., The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse. Hum Mol Genet. 2002 Jul 1;11(14):1647-58
• ID
  MGI:2429322

Genes

Gene	Assay Type
Hook1	PCR

Notes

• Experiment
  The authors used RT-PCR to conduct an expression analysis to determine if a mutation in the Hook1 gene was responsible for azh phenotype. This analysis and subsequent sequencing of the region showed that a 2.021 knb deletion spanning exons 10 and 11 of the Hook1 gene was responsible for the azh phenotype. Western blot analysis confirmed the absence of functional Hook1 protein in azh/azh mice.