Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:79266 Hasegawa M, et al., Progenitor cell mobilization by granulocyte colony-stimulating factor controlled by loci on chromosomes 2 and 11. Blood. 2000 Mar 1;95(5):1872-4
• ID
  MGI:2446650

Genes

Gene	Assay Type
Pbrgcsf1	visible phenotype
D2Mit255	PCR
Hdc

Notes

• Experiment
  Peripheral blood stem cell response to granulocyte colony stimulating factor (G-CSF) differed between C57BL/6J and DBA/2J. In the DBA/2J strain G-CSF mobilized the progenitor cells into the peripheral blood more effectively then did C57BL/6J. The difference in strains enabled the mapping of the quantitative phenotype in the mouse genome. One locus Pbrgcsf1 was identified as mapping with D2Mit255 on mouse Chromosome 2 by using 260 backcross mice derived using strains C57BL/6J and DBA/2J. A possible candidate in the Chromosome 2 QTL region was identified as Hdc, histidine decarboxylase at approximately 71 cM. In addition the BXD RI strains proved useful in identifying a second QTL Pbrgcsf2 on mouse Chromosome 11. Pbrgcsf2 locus was associated with D11Ncvs42. A possible candidate in the Chromosome 11 QTL region was identified as Tcn2, transcobalamin 2 at 3 cMs.