Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  7
• Reference
  J:81444 Zhang Q, et al., Ru2 and Ru encode mouse orthologs of the genes mutated in human Hermansky-Pudlak syndrome types 5 and 6. Nat Genet. 2003 Feb;33(2):145-53
• ID
  MGI:2652612

Genes

Gene	Allele	Assay Type
Sergef		overlapping clones, sequence analysis
Tph1		overlapping clones, sequence analysis
Saa1		overlapping clones, sequence analysis
Saa2		overlapping clones, sequence analysis
Saa3		overlapping clones, sequence analysis
Saa4		overlapping clones, sequence analysis
Hps5	Hps5^ru2	overlapping clones, sequence analysis
Gtf2h1		overlapping clones, sequence analysis
Ldhc		overlapping clones, sequence analysis
Tsg101		overlapping clones, sequence analysis

Notes

• Experiment
  The authors constructed a physical map of the ru2 interval from a contig of 16 overlapping BACs. Three BACs were fully sequenced and several genes were identified. One gene showed obvious abnormalities of the ru2J allele in RT-PCR assays. Further analysis confirmed that mutations in the Hps5 gene were responsible for the ru2 phenotypes. The order of markers in this interval on Chromosome 7 are : 3' - Sergef - 5' - 3'- Tph1 -5' - (Saa1, Saa2, Saa3, Saa4) - 3'- Hps5 - 5' - 5'- Gtf2h1 - 3' - 5'- Ldhc - 3' -3'- Tsg101 - 5'.