Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  1
• Reference
  J:72903 Boettger-Tong HL, et al., Identification and sequencing the juvenile spermatogonial depletion critical interval on mouse chromosome 1 reveals the presence of eight candidate genes. Biochem Biophys Res Commun. 2001 Nov 16;288(5):1129-35
• ID
  MGI:2653537

Genes

Gene	Assay Type
D1Mit213
D1Mit76
D1Mit282
D1Mit181
Utp14b	visible phenotype
D1Mit415
D1Mit94
1700016L21Rik

Notes

• Experiment
  A single homozygous C57BL/6-jsd/jsd female was crossed to a C3H male to obtain B6C3H jsd/+ F1s. These were then intercrossed to obtain F2s. F2 females homozygous for the B6 allele at all loci were selected as homozygous jsd/jsd breeder females and mated with B6C3H F1 jsd/+ males to produce a backcross mapping panel. A total of 31 F2 and 259 backcross males were phenotyped and genotyped. Analysis of the data placed jsd in a 10 cM interval between the markers D1Mit181 and D1Mit415, with a locus order of D1Mit213 - D1Mit76 - D1Mit282 - D1Mit181 - jsd - D1Mit415 - D1Mit94.
  
  An additional 787 backcross males were typed between D1Mit181 and D1Mit415. 20 mice were found to recombinant between these two markers. Additional polymorphic markers were used in this interval to refine the jsd region. Further analysis placed jsd distal to D1Mit215 but proximal to 1700016L21Rik. This represents a genetic distance of 0.4 cM.