Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  16
• Reference
  J:82717 Badalova J, et al., Separation and mapping of multiple genes that control IgE level in Leishmania major infected mice. Genes Immun. 2002 Jun;3(4):187-95
• ID
  MGI:2656578

Genes

Gene	Assay Type
Lmr12	resistance/susceptibility
Lmr12a	resistance/susceptibility
D16Mit126	PCR amplified length variant

Notes

• Experiment
  Susceptibility to Leishmania major infection was analyzed in Recombinant Congenic line CcS/Dem. CcS/Dem RC lines are derived from BALB/cHeA (Leishmania susceptibility) and STS/A (Leishmania resistant). BALB/cHeA exhibits a high IgE serum level after L. major infection, where the STS/A resistant strain has a low level.
  
  CcS/Dem lines carry a random set of 12.5% of genes from the resistant STS/A strain and 87.5% genes from the susceptible BALB/c strain. Two CcS/Dem strains, CcS16 (a high IgE producer) and CcS20 (a low IgE producer) were analyzed for genes that control these levels after Leishmania major infection.
  
  In 578 (BALB/c x CcS16)F2 mice a QTL was associated with D10Mit103 (p <.00000239).
  
  03.10.2016 Curator Note: Table 3 pairs this QTL mapping with D10Mit103 with a previoulsy detected QTL, Lmr5, which also influenced IgE production post infection (J:82715). However, because Lmr5 was originally mapped using a (BALB/c x CcS5)F2 mapping population, which differs from the mapping population used here, we consider this study a separate experiment and have named the QTL, Lmr25.
  
  STS/A alelles at Lmr25 are associated with lower IgE production post infection. The STS/A region of CcS16 contains Stat6, signal transducer and activator of transcription 6, which has been reported to influence IgE levels.
  
  Linkage analysis also detected three additional QTL controlling IgE production in the (BALB/c x CcS16)F2 mapping population:
  Lmr12 on Chr 16, associated with D16Mit126(p <0.00529).
  
  03.29.2016 Curator Note: We have created Lmr12a to reperesnt the QTL influencing IgE production in the current study. Independent QTL have been mapped using this same mapping population to this same locus in J:108764. We are retaining Lmr12 to repesent the collection of novel QTL measured and mapping to this same locus.
  
  Lmr13 on Chr 18, associated with D18Mit35 (p <0.00659); Csf1r, Cd14 and Adrb2 are potential candidate genes for Lmr13 [Table 3.]
  BALB/c alleles at Lmr12a and Lmr13 were asociated with lower IgE production.
  Lmr14 was identified on Chr 2, mapping with D2Mit389, p<0.0160. Lmr14 was identified influencing IgE level in interaction with Lmr25 on mouse Chromosome 10. BALB/c homozygotes at Lmr25 and STS/A homozygotes at Lmr14 or combination of BALB/c homozygtes at these loci resulted in increased IgE levels.