Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  17
• Reference
  J:73737 Morrison SJ, et al., A genetic determinant that specifically regulates the frequency of hematopoietic stem cells. J Immunol. 2002 Jan 15;168(2):635-42
• ID
  MGI:2665082

Genes

Gene	Assay Type
Hscfr	visible phenotype
D17Mit31	PCR amplified length variant
D17Mit51	PCR amplified length variant
D17Mit65	PCR amplified length variant
D17Mit66	PCR amplified length variant
D17Mit39	PCR amplified length variant
D17Mit87	PCR amplified length variant
Notch3	reported elsewhere
Notch4	reported elsewhere

Notes

• Experiment
  Interval mapping was performed on phenotypically extreme animals from a (C57BL/6Ka-Thy1 x AKR/J)F2 intercross to identify QTLs associated with hematopoietic stem cell (HSC) regulation. Parental strain AKR/J exhibits a higher number of long-term reconstituting HSCs compared to parental strain C57BL/Ka-Thy1. A significant locus mapped to approximately 20 cM on mouse Chromosome 17 and is named Hscfr (hematopoietic stem cell frequency regulator). The QTL interval of Hscfr spans 9.8 cM to 33.8 cM, and the peakLOD score is 4.85 at D17Mit65 and D17Mit66. The AKR/J-derived allele is associated with increased HSC frequencies. When an AKR/J-derived region of Hscfr from D7Mit31 to D17Mit87 was bred onto a C57BL/6 background the phenotype was lost. Authors conclude that Hscfr alone is not sufficient to increase HSCs on a C57BL/6 background. Potential candidate genes for Hscfr are Notch3 and Notch4.