Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:80972 Sanchez F, et al., Multigenic control of disease severity after virulent Mycobacterium tuberculosis infection in mice. Infect Immun. 2003 Jan;71(1):126-31
• ID
  MGI:2673457

Genes

Gene	Assay Type
Tbs1	resistance/susceptibility
D3Mit215	PCR amplified length variant

Notes

• Experiment
  Tbs1 and Tbs2 are previously mapped QTLs associated with tuberculosis suvival time. Tbs1 is located on distal mouse Chromosome 3 and Tbs2 is located at 23 cM on mouse Chromosome 9. In the present study the authors extend genotype analysis using 406 (A/SnYCit x I/StSnEgYCit)F2 animals to futher narrow the QTL region and also to investigate linkage to body weight and survival time. Parental strain I/StSnEgYCit exhibits decreased body weight and shorter survival time following infection with Mycobacterium tuberculosis compared to parental strain A/SnYCit. In addition, I/StSnEgYCit females exhibit shorter survival time than I/StSnEgYCit males.
  
  Tbs1 was localized to a 12 cM interval centered on D3Mit215 at 55 cM on mouse Chromosome 3. Females with a heterozygous genotype at Tbs1 exhibit decreased body weight and longer survival times compared to females homozygous for either I/StSnEgYCit-derived or A/SnYCit-derived alleles. Tbs1 appears to be involved in epsitatic interactions with Tbs2 and a locus on mouse Chromosome 17.
  
  Tbs2 was localized to a 9 cM interval centered on D9Mit89 at 8 cM on mouse Chromosome 9. Females with a heterozygous genotype at Tbs2 exhibit decreased body weight and longer survival times compared to females homozygous for either I/StSnEgYCit-derived or A/SnYCit-derived alleles. Body weight loss shows suggestive linkage in male animals, LOD=2.3. Tbs2 overlaps with the Leishmaniasis resistance QTL, Lmr2.
  
  Suggestive linkage to body weight loss and survival time was detected on mouse Chromosome 17 at D17Mit28 (18.44 cM) for males and at D17Mit175 (17.7 cM) for females. This maps relatively close to the H2 locus. Animals homozygous for A/SnYCit-derived alleles at this locus exhibit decreased tuberculosis severity. This locus appears to interact with Tbs1. Females homozygous for A/SnYCit-derived alleles at Tbs1 and homozygous for I/StSnEgYCit-derived alleles at D17Mit175 exhibit the greatest body weight loss.
  
  A locus at DXMit170 also appears to interact with Tbs2 and the chromosome 17 locus. Homozygosity for A/SnYCit-derived alleles at DXMit170 influences body weight loss in conjunction with Tbs2 and D17Mit28/D17Mit175.