Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  5
• Reference
  J:84151 Schneider H, et al., Hypercapnic duty cycle is an intermediate physiological phenotype linked to mouse chromosome 5. J Appl Physiol. 2003 Jul;95(1):11-9
• ID
  MGI:2682395

Genes

Gene	Assay Type
Hycdc	visible phenotype
D5Mit239	PCR amplified length variant
D5Mit7	PCR amplified length variant
D5Mit97	PCR amplified length variant
Dgkq	reported elsewhere
Grk3	reported elsewhere
Nos1	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 69 (C57BL/6J x C3H/HeJ)F2 animals to identify QTLs associated with hypercapnia. Parental strain C57BL/6J exhibits decreased hypercapnic duty cycle at baseline CO(2) concentrations and increased hypercapnic duty cycle at 3% CO(2) compared to parental strain C3H/HeJ. (C57BL/6J x C3H/HeJ)F1 hybrids exhibit increased hypercapnic duty cycle at both baseline and 3% CO(2) compared to both parental strains. Genome scan was performed with 176 microsatellite markers at an average spacing of 10 cM - 15 cM with 90% coverage of the genome.
  
  A QTL associated with hypercapnic duty cycle (Hycdc) mapped to 58 cM on mouse Chromosome 5 with LOD=4.4 at D5Mit239. The QTL interval of Hycdc spans 58 cM - 64 cM with flanking markers D5Mit7 and D5Mit97 defining a broader range. Hycdc explains approximately 30% of the phenotypic variation. Candidate genes mapping near Hycdc include Dgkq, Adrbk2, and Nos1. Sequence variations exist between C57BL/6J and C3H/HeJ for Dgkq. Nos1 knockout animals exhibit abnormal hypoxic ventilation.