Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:86735 Min-Oo G, et al., Pyruvate kinase deficiency in mice protects against malaria. Nat Genet. 2003 Dec;35(4):357-62
• ID
  MGI:3026890

Genes

Gene	Assay Type
Char4	resistance/susceptibility
D3Mit22	PCR amplified length variant
D3Mit75	PCR amplified length variant
D3Mit175	PCR amplified length variant
Pklr	reported elsewhere

Notes

• Experiment
  Recombinant congenic strain AcB55 is resistant to malaria and is derived from parental strains A/J (susceptible) and C57BL/6J (resistant). An F2 cross between AcB55 and A/J was analyzed for linkage to malaria resistance. A locus on mouse Chromosome 3 was detected in linkage to malaria resistance and reticulocytosis and mapped to Char4 between D3Mit22 (33.7 cM) and D3Mit75 (49 cM). Peak linkage to reticulocytosis occurs at D3Mit175 (41 cM). A/J-derived alleles confer recessively-inherited reticulocytosis at this locus.
  
  Pklr is a strong candidate gene mapping near Char4. Sequence analysis of Pklr revealed a loss of function mutation caused by an isoleucine to asparagines substitution at residue 90 (T269A) in the AcB55 strain. This mutation segregated with reduced peak parasitemia and reduced mortality after infection with Plasmodium chabaudi.