Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  7
• Reference
  J:90097 Lundberg YW, et al., Mapping a chromosomal locus for valproic acid-induced exencephaly in mice. Mamm Genome. 2004 May;15(5):361-9
• ID
  MGI:3043720

Genes

Gene	Assay Type
Vpantd	visible phenotype
D7Mit285	PCR amplified length variant
D7Mit101	PCR amplified length variant
Tbx6	reported elsewhere
Prkcb	reported elsewhere
Itgal	reported elsewhere
Itgam	reported elsewhere
Mapk3	reported elsewhere
Plk1	reported elsewhere
Ern2	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 131 (SWV/Fnn x C57BL/6J)F1 x SWV/Fnn backcross fetuses to identify QTLs associated with valproic acid (VPA) induced neural tube defect. Pregnant females were administered VPA at gestational day E8.5 via intraperitoneal injection and fetuses were examined at E15.5-E17.5 via caesarean section. Parental strain SWV/Fnn exhibits high susceptibility to VPA-induced neural tube defect (at a rate of 81.3% exencephaly) whereas parental strain C57BL/6J appears resistant (9.9% exencephaly). 87 polymorphic markers at average intervals of 10 cM-20 cM were screened.
  
  Significant linkage mapped to 44.8 cM on mouse Chromosome 7 near D7Mit285 (p<2x10-6) and is named Vpantd (VPA-induced neural tube defect). This locus spans approximately 6cM and is syntenic to human Chromosome 16p11.2. Candidate genes found within this region include Tbx6, Prkcb, Itgal, Itgam, Mapk3, Plk1, and Ern2. Haplotype analysis mapped the Vpantd critical region between D7Mit285 and D7Mit101. The SWR/Fnn-derived allele appears to be associated with VPA-induced exencephaly.