Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  5
• Reference
  J:87042 Krebs O, et al., Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud. Development. 2003 Dec;130(24):6037-47
• ID
  MGI:3043861

Genes

Gene	Assay Type
Raz	visible phenotype
D5Mit346	PCR amplified length variant
D5Mit391	PCR amplified length variant
D5Mit7	PCR amplified length variant
D5Mit138	PCR amplified length variant
D5Mit348	PCR amplified length variant
D5Mit354	PCR amplified length variant
D5Mit353	PCR amplified length variant

Notes

• Experiment
  The Raz locus was mapped to between 1 cM and 24 cM on mouse Chromosome 5 in a (C3.D2-Raz x C57BL/6)F1 x C57BL/6 backcross. The order of markers is given as follows:
  
  cent - D5Mit346 - Raz - D5Mit391 - D5Mit7 - D5Mit138 - tel
  
  Extensive fine mapping was performed on a population of 553 (C3.D2-Raz x BALB/c)F1 x C3H/HeJ backcross animals. Raz localized to a 16 cM region between D5Mit348 (8 cM) and D5Mit354 (24 cM). This region does not undergo recombination suggesting Raz is located within an inverted segment of DNA. Presence of an inversion was confirmed and the inversion breakpoints were determined to be between D5Mit346 (1 cM) and D5Mit348 (8 cM) at the proximal end and between D5Mit354 (24 cM) and D5Mit353 (24 cM) at the distal end.