About   Help   FAQ
Mapping Data
Experiment
  • Experiment
    TEXT-QTL
  • Chromosome
    4
  • Reference
    J:91543 Wolf N, et al., Quantitative trait locus mapping for age-related cataract severity and synechia prevalence using four-way cross mice. Invest Ophthalmol Vis Sci. 2004 Jun;45(6):1922-9
  • ID
    MGI:3052180
Genes
GeneAlleleAssay TypeDescription
Lnopy1 visible phenotype
D4Mit155 PCR amplified length variant
Tyrp1 reported elsewhere
Foxe3 reported elsewhere
Prdx1 reported elsewhere
Ctrcts visible phenotype
D4Mit55 PCR amplified length variant
D4Mit84 PCR amplified length variant
D4Mit170 PCR amplified length variant
Galt reported elsewhere
Synch1 visible phenotype
Synch2 visible phenotype
Notes
  • Experiment
    Genome scan was performed on 510 animals from a four-way (BALB/cJ x C57BL/6J)F1 x (C3H/HeJ x DBA/2J)F1 cross to identify QTLs associated with age-related cataracts and synechia. Animals were assessed for lens opacity and synechia using slit lamp examination at 18- and 24-months of age. A total of 188 polymorphic loci were genotyped. 96 markers detect maternal alleles (BALB/cJ and C57BL/6J) and 92 markers detect paternal alleles (C3H/HeJ and DBA/2J).

    No linkage was found for lens opacity at 18 months of age. At 24 months of age linkage to lens opacity mapped to 49.6 cM on mouse Chromosome 4 (D4Mit155, P=0.001) and to 29 cM mouse Chromosome 12 (D12Mit34, P=0.28). These QTLs are named Lnopy1 (lens opacity 1) and Lnopy2, respectively. DBA/2J-derived alleleconfers increased lens opacity at Lnopy1 compared to C3H/HeJ, and C57BL/6J-derived alleles confer increased lens opacity at Lnopy2 compared to BALB/cJ. Suggestive linkage to lens opacity mapped to 61 cM on mouse Chromosomes 5 (D5Mit25, P=0.06) and 2.4 cMon mouse Chromosome 11 (D11Mit2, P=0.042). These loci appear to have additive effects. Animals inheriting DBA/2J-derived at Lnopy1, C57BL/6J-derived alleles at Lnopy2, and C3H/HeJ-derived alleles at the chromosome 11 locus exhibit the highest incidence andseverity of cataracts. Potential candidate genes Tyrp1 (38 cM), Foxe3 (49.6 cM), and Prdx1 (47 cM) map to the Lnopy1 interval and play a role in eye disease. Potential candidate gene Chx10 (38 cM) maps to the Lnopy2 interval. Crybb2 (60 cM) is a potentialcandidate gene for the chromosome 5 locus.

    A broad region of mouse Chromosome 4 spanning 20 cM - 67 cM (D4Mit55, D4Mit84, D4Mit170) is associated with cataract severity (P<0.001). This locus is named Ctrcts (cataract severity). DBA/2J-derived alleles confer increased cataract severity compared to C3H/HeJ-derived alleles. Potential candidate gene Galt (20 cM) maps to this region.

    Linkage to synechia in aged mice mapped to two loci on mouse Chromosome 4 at 17 cM (D4Mit55, P=0.001) and 38 cM (D4Mit84, P=0.001) and to a locus on mouse Chromosome 1 at 62 cM (D1Mit493, P=0.006). These loci are named Synch1, Synch2, and Synch3, respectively and appear to act additively. Animals inheriting DBA/2J-derived alleles at Synch3 and Synch1 and BALB/cJ-derived allelesat Synch2 exhibit the highest incidence of synechia. Synechia was observed with higher incidence in aged (24 month old) male mice compared to aged female mice.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/10/2024
MGI 6.24
The Jackson Laboratory