Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  5
• Reference
  J:21289 Tolliver BK, et al., Genetic analysis of sensitization and tolerance to cocaine. J Pharmacol Exp Ther. 1994 Sep;270(3):1230-8
• ID
  MGI:3053927

Genes

Gene	Assay Type
Cyp3a11	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 16 BXD (B=C57BL/6J; D= DBA/2J) recombinant inbred (RI) strains to identify QTLs associated with response to repeated cocaine administration. A panel of 650 polymorphic marker loci were used for QTL mapping. Animals were administered injection of cocaine solution (32 mg/kg) six times daily for 7 days. Parental strain DBA/2J exhibits increased locomotor activity after repeated cocaine administration compared to C57BL/6J. There was no difference in brain cocaine levels between C57BL/6J and DBA/2J after repeated cocaine administration. Loci reported reach the statistical significance level of P<0.01.
  
  Loci associated with cocaine-induced stereotypy (repetitive behavior) mapped to 84 cM on mouse Chromosome 1 near markers Mpmv22 and Pmv21, 46 cM on mouse Chromosome 6 near D6Nds2, and to 18 cM on mouse Chromosome 12 near D12Mit2.
  
  Loci associated with tolerance mapped to 53 cM on mouse Chromosome 5 near marker Cyp3a11 and to 29 cM on mouse Chromosome 8 near D8Mit8.
  
  A locus associated with cocaine-induced locomotor activity (P<0.01) mapped to a region of mouse Chromosome 9 spanning 38 cM - 57 cM near markers D9Mit8 and D9Mit20, respectively. DBA/2J-derived alleles confer increased locomotor activity at this locus.
  
  A locus associated with cocaine-induced locomotor activity mapped to a region of mouse Chromosome 17 spanning 27 cM - 33 cM. Maximum linkage occurred near Hprt-ps1 (47.6 cM, P<0.003). C57BL/6J-derived alleles confer increased locomotor activity at this locus. This locus is also associated with cocaine tolerance where C57BL/6J-derived alleles confer decreased cocaine tolerance.