Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  17
• Reference
  J:73829 van Kooij M, et al., Genotype versus phenotype: conflicting results in mapping a lung tumor susceptibility locus to the G7c recombination interval in the mouse MHC class III region. Immunogenetics. 2001 Oct;53(8):656-61
• ID
  MGI:3055024

Genes

Gene
Vwa7
D17H6S56E-5
Vars1

Notes

• Experiment
  A lung tumor susceptibility locus was previously mapped to the MHC locus on mouse Chromosome 17 around 23 cM. Two congenic strains, B10.A(1R) and B10.A(2R), differ at this locus and were used to study expression differences of potential candidate genes in this region. B10.A(1R) is resistant to chemically induced lung tumors whereas B10.A(2R) is susceptible.
  
  Sequence analysis was performed on 3 genes (D17H6S56E-3, D17H6S56E-5, and Vars2) in a 27 kb interval that differed between B10.A(1R) and B10.A(2R). No SNPs were discovered in this interval except for an A to T change in intron 7 of D17H6S56E-3, which is the breakpoint of the congenic region. Northern blot analysis and quantitative real time PCR did not reveal significant differences in expression between B10.A(1R) and B10.A(2R) at these 3 candidate genes.