Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:87464 Geiger H, et al., Genetic analysis of progenitor cell mobilization by granulocyte colony-stimulating factor: verification and mechanisms for loci on murine chromosomes 2 and 11. Exp Hematol. 2004 Jan;32(1):60-7
• ID
  MGI:3055907

Genes

Gene	Assay Type
Pbrgcsf1	visible phenotype

Notes

• Experiment
  Two previously identified QTLs associated with G-CSF induced stem cell mobilization, Pbrgcsf1 and Pbrgcsf2, were confirmed in this study. Pbrgcsf1 maps to 69 cM on mouse Chromosome 2 and Pbrgcsf2 maps to 4 cM on mouse Chromosome 11. Single and double congenic strains were constructed by introgressing DBA/2J-derived donor DNA at Pbrgcsf1 and/or Pbrgcsf2 onto a C57BL/6J genetic background for 6 to 7 generations. The Pbrgcsf1 congenic interval spans 70 cM - 105 cM and is derived from donor recombinant inbred strain BXD-31. The Pbrgcsf2 congenic interval spans 0 cM - 18 cM and is derived from donor recombinant inbred strain BXD-24. Progenitor strain DBA/2J is a "high responder" to G-CSF induced stem cell mobilization whereas progenitor strain C57BL/6J is a "low responder".
  
  Animals congenic for the Pbrgcsf2 locus at mouse Chromosome 11 exhibit increased stem cell mobilization following G-CSF stimulation whereas animals congenic for the Pbrgcsf1 locus on mouse Chromosome 2 does not. Animals heterozygous for Pbrgcsf1 and/or Pbrgcsf2 exhibit increased stem cell mobilization following G-CSF stimulation. Authors state that these phenotypes are consistent with the original identification of Pbrgcsf1 and Pbrgcsf2.