Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:93071 Kest B, et al., Mapping of a quantitative trait locus for morphine withdrawal severity. Mamm Genome. 2004 Aug;15(8):610-7
• ID
  MGI:3510108

Genes

Gene	Assay Type
Depmq1	visible phenotype
D1Mit48	PCR amplified length variant
Plcd4	reported elsewhere
Ugt1a1	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on a population of (C57BL/6J x 129P3/J)F2 intercross animals to identify QTLs associated with morphine withdrawal. 87 polymorphic markers were genotyped in an initial panel of 124 F2 animals. An additional 100 F2 animals were genotyped to refine the candidate locus. Parental strain C57BL/6J exhibits 10-fold greater morphine withdrawal jumping frequency compared to parental strain 129P3/J. Animals were administered morphine injection three times a day over 4 days and withdrawalwas induced with nalaxone on day 5.
  
  Significant linkage mapped to an interval on mouse Chromosome 1 spanning 32 cM - 60 cM. This locus is named Depmq1 (dependence on morphine QTL 1). Depq1 reaches peak linkage at 51 cM near D1Mit48 with LOD=4.7. C57BL/6J-derived alleles confer increased withdrawal jumping frequency with a recessive mode of inheritance. Depmq1 accounts for 20% of the phenotypic variance. Depmq1 maps near a previously identified QTL for locomotor activity named Hdis at 54 cM on mouse Chromosome 1. Potential candidate genes mapping near Depmq1 are Plcd4 (39.2 cM) and Ugt1a1 (51.7 cM).
  
  Suggestive loci mapped to 24 cM (LOD=2.3) on mouse Chromosome 5 and 45 cM (LOD=2.9) on mouse Chromosome 10.
  
  Depmq1 combined with the 2 suggestive QTLs account for 43% of the phenotypic variance. Pairwise scanning did not detect interaction among the 3 loci.