Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:92935 de Souza CM, et al., Quantitative trait loci in Chromosomes 3, 8, and 9 regulate antibody production against Salmonella flagellar antigens in the mouse. Mamm Genome. 2004 Aug;15(8):630-6
• ID
  MGI:3510710

Genes

Gene	Assay Type
Ssta5	visible phenotype
D3Mit100	PCR amplified length variant
Il12a	reported elsewhere
Il6ra	reported elsewhere
Fcgr1	reported elsewhere

Notes

• Experiment
  
  Genome scan was used to identify QTLs associated with antibody response regulation against infection with Salmonella typhimurium. 146 microsatellite markers were typed in 60 phenotypically extreme F2 animals from a (HIII x LIII)F2 intercross. The markers are spaced at an average of 9 cM and with 85% genome coverage Animals were injected twice intraperitoneally with 3.3 x 10⁹ inactivated S.typhimurium at a 7 day interval. HIII is a high antibody responder strain whereas LIII is a low antibody responderstrain. Three novel QTLs reaching statistical significance of LRS>23 were identified.
  
  Ssta5 (susceptibility to Salmonella typhimurium antigens 5) mapped to 49 cM on mouse Chromosome 3 near D3Mit100 (LRS=24.3). The 95% confidence interval of Ssta5 spans 27 cM. Potential candidate genes Il12a (37 cM), Il6ra (42.1 cM), and Fcgr1 (45.2 cM) map near Ssta5.
  
  Ssta6 mapped to 38.5 cM on mouse Chromosome 8 near D8Mit249 (LRS=24.6). The 95% confidence interval of Ssta6 spans 26 cM. Potential candidate gene Il12rb1 (33.5 cM) maps near Ssta6.
  
  Ssta7 mapped to 34 cM on mouse Chromosome 9 near D9Mit248 (LRS=28.5). The 95% confidence interval of Ssta7 spans 23 cM. Potential candidate genes Il10ra (26 cM), Il18 (29 cM), and the Cd3 cluster (26 cM) map near Ssta7.
  
  Suggestive loci mapped to 80 cM on mouse Chromosome 5 near D5Mit292, 40 cM on mouse Chromosome 10 near D10Mit198, 58 cM on mouse Chromosome 12 near D12Mit19, and 19 cM on mouse Chromosome 13 near D13Mit117.