Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  X
• Reference
  J:92309 Storchova R, et al., Genetic analysis of X-linked hybrid sterility in the house mouse. Mamm Genome. 2004 Jul;15(7):515-24
• ID
  MGI:3511516

Genes

Gene	Assay Type
Hstx1	visible phenotype
DXMit119	PCR amplified length variant
DXMit60	PCR amplified length variant
Ihpd	reported elsewhere

Notes

• Experiment
  Animals consomic for a PWD/Ph-derived X chromosome were created by backcrossing F1 hybrids to background strain C57BL/6J and selecting for non-recombinant PWD/Ph-derived X chromosomes at each generation. Consomic male animals C57BL/6J- Chr X< PWD/Ph> Y^C57BL/6J are fully sterile whereas consomic female animals C57BL/6J- Chr X< PWD/Ph> X^C57BL/6J are fertile. Histological sections of testicular tissue from consomic males were abnormal and the sperm heads also appeared abnormal. 11 microsatellite markersalong the length of the X chromosome were genotyped in 346 (C57BL/6J- Chr X< PWD/Ph> X^C57BL/6J x C57BL/6J)N7 x C57BL/6J backcross animals to identify X-linked fertility QTLs.
  
  An important factor in X-linked sterility mapped to 29.5 cM near DXMit119. The PWD/Ph-derived allele confers male sterility at this locus, named Hstx1 (hybrid sterility, X chromosome 1). The peak LRS value of Hstx1 reached 112.7 between the 99% confidence interval DXMit119 (29.5 cM) and DXMit60 (30.8 cM). This locus also shows maximal linkage to abnormal sperm head morphology (LRS=69.7), testis weight (LRS=73.6), and sperm count (LRS=30). Ihtw1 (18 cM) is a testicular weight QTL mapping near the Hstx1 interval. Ihpd is a placental incompatibility gene mapping near Hstx1.
  
  Hstx1 is necessary but not sufficient for sterility and may interact with at least one other X-linked factor. An interval between DXMit173 (50.5 cM) and DXMit234 (58 cM) shows linkage to testis weight and sperm count.