Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  14
• Reference
  J:94208 Bannister LA, et al., Positional cloning and characterization of mouse mei8, a disrupted allele of the meiotic cohesin Rec8. Genesis. 2004 Oct 27;40(3):184-194
• ID
  MGI:3521570

Genes

Gene	Assay Type	Description
Rec8	mutagenesis	mei8
D14Mit234	PCR
D14Mit258	PCR
D14Mit25	PCR

Notes

• Experiment
  A mutant (mei8) induced in v6.4 ES cells from mouse hybrid strain (C57BL/6J x 129S4/Jae) was genetically mapped to mouse Chromosome 14. Male chimeric mice transmitting mei8 were outcrossed to CAST/Ei females,the resulting F1 progeny were intercrossed andbackcrossed. Recombination mapping showed that mei8 was located in a genetic region between D14Mit234 and D14Mit258 (21 recombinants per 458 meioses analyzed). One recombination event (out of 458 meioses analyzed) further refined the genetic interval to a region between D14Mit25 and D14Mit258. This region included Rec8L1.