Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:94152 Yi N, et al., Epistatic interaction between two nonstructural loci on chromosomes 7 and 3 influences hepatic lipase activity in BSB mice. J Lipid Res. 2004 Nov;45(11):2063-70
• ID
  MGI:3521777

Genes

Gene	Assay Type
Hlbsb2	visible phenotype
D3Mit18	PCR amplified length variant

Notes

• Experiment
  A population of 5 month old BSB backcross (B=C57BL/6J; S=Spret/Pt) animals (n=203) were examined for linkage to hepatic lipase activity. 148 polymorphic markers covering 90% of the genome at an average resolution of 10 cM were used in the genome scan.
  
  Hlbsb1 (hepatic lipase activity in BSB 1) mapped to 64 cM on mouse Chromosome 7 with a peak LOD score of 4.8 near D7Mit187. Hlbsb1 accounts for approximately 10.67% of the phenotypic variance. The QTL interval of Hlbsb1 spans approximately 48 cM - 66 cM. SPRET/Pt-derived alleles confer increased hepatic lipase activity at Hlbsb1. Hlbsb1 maps near a previously identified obesity/HDL/hepatic lipase QTL named Mob1 (62 cM).
  
  A second interacting locus was identified on mouse Chromosome 3 at 66.4 cM near D3Mit18 and is named Hlbsb2. SPRET/Pt-derived alleles confer increased hepatic lipase activity at Hlbsb2 in epistatic interactions with Hlbsb1. Animals with SPRET/Pt-derived alleles at both Hlbsb1 and Hlbsb2 exhibit enhanced hepatic lipase activity. The interaction between Hlbsb1 and Hlbsb2 explains approximately 7.03% of the phenotypic variance.