Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  7
• Reference
  J:94250 Megumi T, et al., Mapping of putative ether-anesthesia resistance gene using C57BL/6J and MSM/Ms mouse strains. J Anesth. 2004;18(4):267-76
• ID
  MGI:3522105

Genes

Gene	Assay Type
Etan1	resistance/susceptibility
D7Mit313	PCR amplified length variant
D7Nds1	PCR amplified length variant
Gabrg3	reported elsewhere
Gabra5	reported elsewhere
Gabrb2	reported elsewhere
Chrna7	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 196 (C57BL/6J x MSM/Ms)F1 x MSM/Ms backcross animals to identify QTLs influencing sensitivity to ether anesthesia. Parental strain C57BL/6J exhibits resistance to ether anesthesia whereas parental strain MSM/Ms is sensitive. (C57BL/6J x MSM/Ms)F1 hybrids are resistant to ether anesthesia. An initial screen was done with 40 microsatellite markers using 12 resistant and 12 sensitive backcross animals. Linkage was detected on mouse Chromosomes 3, 7, and 8. More markers weretyped in the remaining animals and mouse Chromosome 7 showed the strongest linkage between 23 cM (D7Mit313) and 29 cM (D7Nds1). Peak linkage occurred near D7Nds1 with LOD=3.03. This locus is named Etan1 (ether anesthesia 1). Animals heterozygous at Etan1 are resistant to ether anesthesia compared animals homozygous for MSM/Ms-derived alleles. Potential candidate genes mapping near Etan1 are Gabrg3 (28.2 cM), Gabra5 (28.5 cM), Gabrb2 (28.6 cM), and Chrna7 (30 cM).