Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  8
• Reference
  J:88564 Turcotte K, et al., Genetic control of myeloproliferation in BXH-2 mice. Blood. 2004 Mar 15;103(6):2343-50
• ID
  MGI:3574549

Genes

Gene	Assay Type
Irf8	visible phenotype
D8Mit13	PCR
D8Mit14	PCR
D8Mit200	PCR

Notes

• Experiment
  The recombinant inbred strain BXH2/TyJ exhibits splenomegaly, enlarged lymph nodes, and susceptibility to infection with Mycobacterium bovis. This phenotype is not seen in progenitor strains C57BL/6J and C3H/HeJ, so it appears to be unique to BXH2/TyJ. This phenotype also does not co-segregate with Slc11a1, which is involved in immune response to infection with various microorganisms. Splenomegaly is accompanied by histopathological abnormalities in the spleen, bone marrow, and lymph nodes. Increased levels of Mac1 and GR1 cell types was also observed with splenomegaly. Several crosses involving BXH2/TyJ and A/J or BALB/cJ show that splenomegaly segregates as a recessive single locus trait and is not dependent on infection with M. bovis. Linkage analysiswas performed using 193 polymorphic markers at an average spacing of 10 cM.
  
  Highly significant linkage to splenomegaly mapped to central mouse Chromosome 8 and is named Myls (myeloproliferative syndrome). 187 (BALB/cJ x BXH2/TyJ)F2 animals infected with M. bovis and 118 uninfected F2 animals were analyzed. Strongest linkage was at D8Mit13 (67 cM) in both populations (LOD=44.1 infected; LOD=34.5 uninfected). 187 (A/J x BXH2/TyJ)F2 animals infected with M. bovis and 91 uninfected F2 animals were also analyzed and confirmed Myls. Strongest linkage was at D8Mit14 (67 cM) in both populations (LOD=24.6 infected; LOD=24.9 uninfected). The Myls candidate region is an 18 cM interval flanked by D8Mit200 (58 cM) and D8Mit13 (67 cM).