Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:96394 Leder A, et al., zeta-/- Thalassemic mice are affected by two modifying loci and display unanticipated somatic recombination leading to inherited variation. Hum Mol Genet. 2005 Mar 1;14(5):615-25
• ID
  MGI:3574912

Genes

Gene	Assay Type
Gstml	visible phenotype
D2Mit48	PCR

Notes

• Experiment
  Animals homozygous for the Hba-x^tm1Led mutation on a 129S/SvEv genetic background do not survive gestation. However, homozygous mutants on a C57BL/6J genetic background do survive gestation. In order to map genes modifying embryonic survival approximately 115 polymorphic markers were genotyped in 97 Hba-x^tm1Led homozygous (129S/SvEv-Hba-x^tm1Led x C57BL/6J)F1 x 129S/SvEv-Hba-x^tm1Led backcross animals and 105 littermate controls.
  
  Linkage to embryonic survival mapped to 87 cM on mouse Chromosome 2near D2Mit48 (P<0.0014) and 70 cM on mouse Chromosome 5 near D5Mit161 (P<0.032). The chromosome 2 locus is named Gstml (gestational survival modifier locus). The chromosome 5 locus did not reach statistical criteria to declare linkage. C57BL/6J-derived alleles confer increased embryo survival at the chromosome 2 and chromosome 5 loci. Interestingly, these loci are also associated with loss of heterozygosity (LOH) in surviving homozygous mutant animals.