Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:97046 Zucchelli S, et al., Defective Central Tolerance Induction in NOD Mice: Genomics and Genetics. Immunity. 2005 Mar;22(3):385-396
• ID
  MGI:3575700

Genes

Gene	Assay Type
Tcdel2	visible phenotype
Naa15	reported elsewhere
Ash1l	reported elsewhere
Sh2d2a	reported elsewhere
Cd1d1	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 306 (C57BL/6-H2^g7 x NOD/LtDOI)F2 embryos (transgenic for Tg(TcraBDC2.5)1Doi and Tg(TcrbBDC2.5)2Doi) to identify QTLs associated with T cell clonal deletion. 147 SNP markers spanning 19 autosomes and spaced approximately 16 Mb apart were used for the genome scan. Parental strain NOD/LtDOI exhibits resistance to T cell clonal deletion compared to parental strain C57BL/6-H2^g7.
  
  Significant linkage to %DP (double positive) and %DN (double negative) T cells mapped to 135 Mb on mouse Chromosome 1 (LOD=4.2 for %DP; LOD=5.51 for %DN). This locus explains 15.2% of the variance and is named Tcdel1 (T cell clonal deletion 1). NOD/LtDOI-derived alleles at Tcdel1 confer increased %DP and decreased %DN T cells in non-peptide supplemented cell cultures derived from F2 embryos with a dominant mode of inheritance. Potential candidate genes mapping near Tcdel1 are Pdcd1, Bcl2 (59.8 cM), and Ptprv. Pdcd1 and Bcl2 exhibit differential expression in cell cultures derived from NOD/LtDOI and C57BL/6-H2^g7 mice. Previously identified QTLs Idd5a (38.5 cM) and Idd5b (41 cM) map near Tcdel1.
  
  Significant linkage to %DP and %DN T cells from F2-derived cultures using peptide-supplemented media mapped to mouse Chromosome 3 (LOD=14.2). This locus explains 31.2% of the variance and is named Tcdel2 (T cell clonal deletion 2). NOD/LtDOI-derived alleles at Tcdel2 confer increased %DP and decreased %DN T cells in peptide supplemented cell cultures derived from F2 embryos with a dominant mode of inheritance. Potential candidate genes are Narg1, Ash1l, Sh2d2a (42.6 cM), and Cd1d1 (48 cM). Previously identified QTL Idd17 (39 cM) overlaps with the Tcdel2 interval. Idd3 (19.2 cM) and Idd10 (48.5 cM) map to either side of the Tcdel2 interval.
  
  Linkage to %CD8-SP (single positive) T cells mapped to central mouse Chromosome 7 (LOD=9.69). This locus is named Cspt (CD8 single positive T cells). The QTL interval is very broad, spanning approximately 20 Mb to 110 Mb. C57BL/6-derived alleles at Cspt confer increased %CD8-SP T cells with a dominant mode of inheritance.