Experiment
Novel SNPs were identified and used to fine map the Pas1 locus. 29 inbred strains were genotyped to isolate Pas1 to a 486 kb region on mouse Chromosome 6. Several of these SNP markers are located within one of the Pas1 candidate genes.
D6Int4, D6Int32a, D6Int32b, D6Int32c, D6Int33, D6Int54, and D6Int8 are located in Lrmp.
D6Int37a, D6Int38, D6Int84, D6Int40, D6Int95, D6Int96, D6Int41, D6Int101, and D6Int102 are located in Casc1.
D6Int44, D6Int126, D6Int127, D6Int128, D6Int130, D6Int131, D6Int134, D6Int135, D6Int137, D6Int42, and D6Int10 are located in 4930469P12Rik (Ghiso).
D6Int142, D6Int143, and D6Int145 are located in Kras.
D6Int46, D6Int47, D6Int56, and D6Int153 are located in 4933403M22Rik (Lmna-rs1).
The order of the SNP markers is as follows:
centromere - D6Int30 - D6Int4 - D6Int32a - D6Int32b - D6Int32c - D6Int33 - D6Int54 - D6Int8 - D6Int37a - D6Int38 - D6Int84 - D6Int40 - D6Int95 - D6Int96 - D6Int41 - D6Int101 - D6Int102 - D6Int44 - D6Int126 - D6Int127 - D6Int128 - D6Int130 - D6Int131 - D6Int134 - D6Int135 - D6Int137 - D6Int42 - D6Int10 - D6Int142 - D6Int143 - D6Int145 - D6Int43 - D6Int146 - D6Int46 - D6Int47 - D6Int56 - D6Int153 - telomere