Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:97940 Yamaji K, et al., Evidence for Cd101 but not Fcgr1 as candidate for type 1 diabetes locus, Idd10. Biochem Biophys Res Commun. 2005 Jun 3;331(2):536-42
• ID
  MGI:3582436

Genes

Gene	Assay Type
Idd10	resistance/susceptibility
D3Mit231	PCR
D3Mit292	PCR
Igsf3	reported elsewhere
Fcgr1	reported elsewhere

Notes

• Experiment
  Examination of the NOD-related strains revealed a naturally occurring recombination of the Idd10 (48.5 cM) locus on mouse Chromosome 3 in IIS/Shi. The Fcgr1 sequence at 45.2 cM in inbred strain IIS/Shi is derived from NOD/Shi while the flanking regions are derived from IIS/Shi. Fcgr1 has been proposed as a candidate gene for Idd10 as the NOD allele of Fcgr1 is missing 75% of the cytoplasmic domain and results in a defective gene product. Igsf3 (formerly Cd101) at 48.5 cM is another Idd10 candidate gene located outside the NOD/Shi-derived region.
  
  A congenic line named NOD.IIS-Idd10 was constructed by introgressing a 35 cM IIS/Shi-derived donor region between D3Mit231 (38.3 cM) and D3Mit292 (73.9 cM) onto the NOD/Shi genetic background. (Note that the Fcgr1 sequence in the congenic is derived from NOD/Shi while the Igsf3 sequence is derived from IIS/Shi.) The NOD.IIS-Idd10 congenic exhibits resistance to type 1 diabetes compared to susceptible background strain NOD/Shi. The IIS/Shi-derived Idd10 congenicregion confers dominantly-inherited diabetes resistance. Since the congenic Fcgr1 sequence is derived from NOD/Shi this gene does not appear to have an effect on diabetes and was excluded as a candidate. The Idd10 locus on mouse Chromosome 3 is possibly divided into 3 different components: Idd10 (48.5 cM), Idd17 (39 cM), and Idd18 (53.3 cM). The NOD.IIS-Idd10 congenic contains all 3 loci.
  
  Sequence analysis revealed that the IIS/Shi sequence of Igsf3 is identical to that of C57BL/6J, another diabetes-resistant strain. The Igsf3 sequence differed between IIS/Shi and NOD/Shi at 17 SNPs, 10 of which result in amino acid substitutions. Igsf3 remains an attractive candidate gene for Idd10.