Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  X
• Reference
  J:99479 Oliver F, et al., Regulatory variation at glypican-3 underlies a major growth QTL in mice. PLoS Biol. 2005 May;3(5):e135
• ID
  MGI:3582716

Genes

Gene	Assay Type
Bw19	visible phenotype
DXMit226	PCR
DXMit68	PCR
Gpc3	reported elsewhere
Gpc4	reported elsewhere
Magea14	reported elsewhere
A630012P03Rik	reported elsewhere

Notes

• Experiment
  A previously identified body weight QTL named Bw19 was further refined to a 660 kb interval on mouse Chromosome X between DXMit226 (16.1 cM) and DXMit68 (17.25 cM) by progeny testing. Interval specific congenic animals derived from HI (high body weight) and LI (low body weight) lines were used to fine map this locus. Several candidate genes are found in this region: Gpc3, Gpc4, 1700080O16Rik, and A630012P03Rik.
  
  Several polymorphisms were detected for Gpc3 between the HI and LI lines. The 3' UTR polymorphisms in Gpc3 are considered strong candidates for the effects observed at Bw19. Animals with HI-derived alleles at Gpc3 exhibit 15% lower expression in liver and kidney compared to animals with LI-derived alleles. Gpc3 knockout animals exhibit increased body mass, renal dysplasias, and increased perinatal mortality. In addition, loss-of-function GPC3 mutations in humans leads to a disease with similar phenotypes called Simpson-Golabi-Behmel syndrome.
  
  Examination of liver and kidney Gpc4 mRNA did not reveal significant differences in gene expression between HI and LI animals.