Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:99373 Klein OF, et al., Confirmation and fine mapping of chromosomal regions influencing peak bone mass in mice. J Bone Miner Res. 2001 Nov;16(11):1953-61
• ID
  MGI:3583581

Genes

Gene	Assay Type
Bomd3	visible phenotype
D2Mit480	PCR
D2Mit94	PCR
Ext2	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on a population of (C57BL/6J x DBA/2J)F2 animals to confirm provisional QTL associated with bone mineral density (BMD) mapped in a previous study. A subset of phenotypically extreme F2 animals was genotyped for an initial genetic screen. Next, all 994 F2 animals were genotyped for markers flanking provisional QTL. All identified QTL were also confirmed using short-term selected lines by comparing allele frequencies at each identified locus in high BMD or low BMD lines. FourQTL accounting for 16% of the variance in peak whole body BMD were identified.
  
  Bomd5 (bone mineral density 5) mapped to 101.5 cM on mouse Chromosome 1 near D1Mit291 (LOD=6.2). DBA/2J-derived alleles at Bomd5 confer increased whole body bone mineral density. Bmd1 (87.9 cM) is a previously identified bone density QTL mapping near Bomd5. The Bomd5 interval is syntenic to human Chromosome 1q21-q43.
  
  Bomd3 (bone mineral density 3) mapped to 51.4 cM on mouse Chromosome 2 near D2Mit480. DBA/2J-derived alleles at Bomd3 confer increased whole body bone mineral density. Bomd3 occupies a broad interval so recombinant inbred strain analysis was used to localize the QTL. Recombinant inbred strain BXD-8 carries a recombination between D2Mit14 (C57BL/6J-derived) and D2Mit249 (DBA/2J-derived). Association between BMD and a marker (D2Mit94; LOD=11) proximal to the recombination point was observed in a (BXD-8 x DBA/2J)F2 intercross. This result narrows the Bomd3 region to a 9-10 cM interval around 48 cM. A potential candidate gene for Bomd3 is Ext2 at 51 cM. Sbmd2 (18 cM) is a previously identified bone density QTL mapping near Bomd3. The Bomd3 interval is syntenic to human Chromosome 2q21-q32 and human Chromosome 11p-q12.
  
  Bomd6 (bone mineral density 6) mapped to 69.8 cM on mouse Chromosome 4 near D4Mit312 (LOD=12.3). DBA/2J-derived alleles at Bomd6 confer increased whole body bone mineral density. The Bomd6 interval is syntenic to human Chromosome 1p36.3-p34.1.
  
  Bomd7 (bone mineral density 7) mapped to 52 cM on mouse Chromosome 11 near D11Mit179. This locus may contain 2 closely linked QTL. C57BL/6J-derived alleles at Bomd7 confer increased whole body bone mineral density. Bomd7 occupies a broad interval so recombinant inbred strain analysis was used to localize the QTL. Recombinant inbred strain BXD-8 carries a recombination between D11Mit355 (C57BL/6J-derived) and D11Mit179 (DBA/2J-derived). Association between BMD and a marker (D11Mit349; LOD=10.1) proximal to the recombination point was observed in a (BXD-8 x DBA/2J)F2 intercross. This result reduces the Bomd7 interval to a region around 32 cM containing several candidate genes. Sbmd4 (49 cM) is a previously identified bone density QTL mapping near Bomd7. The Bomd7 interval is syntenic to human Chromosome 5q31-q35and human Chromosome 17p13-q22.