Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  3
• Reference
  J:61763 Lyons PA, et al., Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping. Genome Res. 2000 Apr;10(4):446-53
• ID
  MGI:3583619

Genes

Gene	Assay Type
Idd3	resistance/susceptibility
D3Nds6	PCR
Il2	reported elsewhere

Notes

• Experiment
  The Idd3 locus on mouse Chromosome 3 was narrowed to a 145 kb interval using a combination of congenic strain haplotype analysis, SNP haplotype analysis, and contig mapping. The Idd3 interval is most likely located between D3Nds6 (19.2 cM) and SNP 81.3. Il2 is a strong candidate mapping to this interval. Sequence analysis of the Il2 promoter region between diabetes susceptible and resistant mouse strains revealed 2 polymorphisms in non-regulatory regions. Furthermore, Il2 expression levels did not differbetween diabetes susceptible and resistant strains. A serine to proline amino acid substitution at residue 6 of the Il2 protein is associated with increased glycosylation and diabetes susceptibility. This non-conservative polymorphism may account for theeffect of Idd3.