Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  18
• Reference
  J:98656 Wang M, et al., Analysis of the Par2 modifier of pulmonary adenoma formation in mice. Exp Lung Res. 2005 Mar;31(2):193-204
• ID
  MGI:3586640

Genes

Gene	Assay Type
Par2	resistance/susceptibility
D18Mit103	PCR
D18Mit188	PCR
Dynap	reported elsewhere
Stard6	reported elsewhere
Poli	reported elsewhere
Mbd2	reported elsewhere
Dcc	reported elsewhere

Notes

• Experiment
  In a previous study, the Par2 (pulmonary adenoma resistance 2) locus was localized to a 2.4 Mb interval between D18Mit103 (44 cM) and D18Mit188 (47 cM). The current study examines possible candidate genes found within the Par2 critical region. These genes are 2310002L13Rik, Stard6 (44 cM), Poli, Mbd2 (44 cM), and Dcc (45 cM). Poli shows the strongest evidence as a Par2 candidate gene. Sequence analysis revealed 25 nucleotide polymorphisms between lung tumor susceptible strain A/J and lung tumor resistantstrain BALB/cJ resulting in 10 amino acid differences. Two different Poli alternative transcripts (exon 4a and exon 2d) were also observed in lung mRNA of A/J and BALB/cJ animals. The exon 4a isoform encodes a truncated protein due to a premature stop codon, and the exon 2d isoform results in the splicing out of exon 2 without disruption of the reading frame. Resistant strain BALB/cJ produces decreased levels of functional Poli protein due to increased expression of the exon 4a isoform. The Poli protein also displays functional differences and substrate preference between A/J and BALB/cJ strains.