Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  10
• Reference
  J:99472 Ferraro TN, et al., Confirmation of a major QTL influencing oral morphine intake in C57 and DBA mice using reciprocal congenic strains. Neuropsychopharmacology. 2005 Apr;30(4):742-6
• ID
  MGI:3589120

Genes

Gene	Assay Type
Mop2	visible phenotype
D10Mit75	PCR
D10Mit124	PCR
D10Mit61	PCR
Oprm1	reported elsewhere

Notes

• Experiment
  This study confirms the presence of a major morphine preference QTL on proximal mouse Chromosome 10 named Mop2. Reciprocal congenic lines derived from inbred strains C57BL/6J and DBA/2J were constructed. Parental strain C57BL/6J has a high preference formorphine compared to parental strain DBA/2J.
  
  Congenic line B6.D2-Mop2 contains DBA/2J-derived DNA covering markers D10Mit75 (2 cM) to D10Mit124 (15 cM) on a C57BL/6J genetic background. This line exhibits significantly decreased morphine consumption compared to background strain C57BL/6J.
  
  Congenic line D2.B6-Mop2 contains C57BL/6J-derived DNA covering markers D10Mit75 (2 cM) to D10Mit61 (32 cM). This line exhibits significantly increased morphine consumption compared to background strain DBA/2J.
  
  Thisstudy localizes Mop2 to an interval on proximal mouse Chromosome 10 between the acromere and D10Mit124 (15 cM).
  
  Oprm1 (8 cM) is considered a strong candidate gene for Mop2. Homozygous Oprm1 knockout animals appear insensitive to the analgesic effects ofmorphine.