Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  4
• Reference
  J:67572 Zhang SL, et al., Efficiency alleles of the Pctr1 modifier locus for plasmacytoma susceptibility. Mol Cell Biol. 2001 Jan;21(1):310-8
• ID
  MGI:3609335

Genes

Gene	Assay Type
Cdkn2a	resistance/susceptibility
D4Mit15	PCR amplified length variant

Notes

• Reference
  Gene symbol pctr1 has been withdrawn and is now an allele of Cdkna2.
• Experiment
  Previously identified plasmacytoma modifier locus Pctr1 (plasmacytoma resistance 1) was narrowed to a 1.5 cM interval on mouse chromosome 4 using a congenic line carrying DNA spanning Cdkn2a (42.7 cM) to D4Mit15 (42.6 cM) from resistant donor strain DBA/2 on a BALB/c susceptible background. This line is called C.D2-(Cdkn2a-D4Mit15)/Lgm and is estimated to contain 1.5 cM of DBA/2 DNA (which encompasses the Pctr1 locus) on an otherwise BALB/c background. This line is significantly more resistant to plasmacytoma development compared to BALB/c.
  
  The authors propose Cdkn2a as a candidate gene for Pctr1. Inbred strain C57BL/6 is resistant to pristine-induced plasmacytomas but animals homozygous for the Cdkn2a^tm2.1Rdp knockout allele are susceptible and display accelerated pristine-induced plasmacytomagenesis. In addition, the Cdkn2a sequence contains polymorphisms P18H and I51V in the p16INK4a protein and H72R in the p19ARF protein between susceptible strain BALB/cAnPt and resistant strain DBA/2N, respectively. Overexpression of the BALB/c allele of Cdkn2a appears much less efficient in arresting cell growth of plasmacytoma cell lines in vitro compared to the DBA/2 allele of Cdkn2a.