Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  6
• Reference
  J:108212 Galbiati F, et al., Allelic effects of mouse Pas1 candidate genes in human lung cancer cell lines. Cancer Lett. 2006 Dec 8;244(2):176-81
• ID
  MGI:3623880

Genes

Gene	Assay Type
Pas15	resistance/susceptibility
Irag2	reported elsewhere
Dnai7	reported elsewhere
Etfrf1	reported elsewhere
Lmntd1	reported elsewhere

Notes

• Experiment
  Previously identified QTL Pas15 (pulmonary adenoma susceptibility 1) maps to a 468 kb interval on mouse Chromosome 6. This region contains six candidate genes: Lrmp, Casc1, 4930469P12Rik (aka Ghiso), and 4933403M22Rik (aka Lmna-rs1). Allele-specific expression constructs of each of the candidate genes were derived from lung mRNA from inbred strains A/J (pulmonary adenoma susceptible) and C57BL/6J (pulmonary adenoma resistant). The expression constructs were transfected into 2 human small-cell lung carcinoma cell lines and assayed for transformation. The ability of Lrmp and 4930469P12Rik constructs to transform human lung carcinoma cells did not differ between A/J and C57BL/6J alleles. However, the A/J allele of Casc1 produced fewer transformed human cells compared to the C57BL/6J allele, and the A/J allele of 4933403M22Rik produced more transformed human cells compared to the C57BL/6J allele. Casc1 was observed preferentially expressed in lung tumors of the A/J-derived allele. 4933403M22Rik expression was not observed in mouse lung tumors of any genotype. Authors state that the experimental results do not necessarily preclude Lrmp and 4930469P12Rik as candidate genes for Pas1.