Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  9
• Reference
  J:108764 Havelkova H, et al., Genetics of susceptibility to leishmaniasis in mice: four novel loci and functional heterogeneity of gene effects. Genes Immun. 2006 Apr;7(3):220-33
• ID
  MGI:3625975

Genes

Gene	Assay Type
Lmr17	visible phenotype
D9Mit2	PCR amplified length variant

Notes

• Experiment
  Disease phenotypes associated with Leishmania major infection were mapped in animals from (CcS-16 x BALB/cHeA)F2 and (CcS-20 x BALB/cHeA)F2 intercross populations. CcS-16 and CcS-20 are recombinant congenic (RC) strains derived from STS/A and BALB/cHeA. Animals were infected with Leishmania major after 9 weeks of age and sacrificed 8 weeks post-infection.
  
  F2 hybrids between BALB/c and CcS20/Dem and F2 hybrids between BALB/c and CcS16 were tested from clinical and immunological consequences post infection. They were genotyped with mircosatellite markers covering the STS-derived segments. Linkage analysis of differing pathological and immunological parameters indicated novel loci and novel functions for previously mapped loci.
  
  In the (BALB/c x CcS20)F2 intercross a novel QTL, Lmr17 mapped with marker D9Mit2, corrected p=0.0107. STS/A alleles at this locus were associated with increased levels of TNF alpha in serum.
  
  Lmr24f, mapping to D5Mit175, was identified in interaction with Lmr17 at D9Mit2 influencing IFN gamma, corrected p=0.0046. Homozygote BALB/c alleles at Lmr24f and homozygous STS/A alleles at Lmr17 (D9Mit2) increased IFN gmma levels.