Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  1
• Reference
  J:108692 Angers I, et al., Tlr5 is not primarily associated with susceptibility to Salmonella Typhimurium infection in MOLF/Ei mice. Mamm Genome. 2006 May;17(5):385-97
• ID
  MGI:3654077

Genes

Gene	Assay Type
Ity3	resistance/susceptibility
Tlr5	reported elsewhere

Notes

• Experiment
  Congenic animals derived from resistant C57BL/6J and susceptible MOLF/Ei parentals were used to test the candidacy of Tlr5 for the Salmonella susceptibility QTL Ity3 (71.5 cM on chromosome 1). Introgression of MOLF/Ei-derived Ity3 onto a C57BL/6J geneticbackround included the Tlr5 locus. The following parentals and congeic lines were tested by injection with flagellin:
  
  C57BL/6J
  MOLF/Ei
  B6.MOLF-Slc11a1 Ity3 (homozygous for MOLF/Ei alleles at Tlr5)
  B6.MOLF-Slc11a1 Ity3^B6/MOLF (heterozygous for C57BL/6J and MOLF/Ei alleles at Tlr5)
  B6.MOLF-Slc11a1 (homozygous for C57BL/6J alleles at Tlr5)
  
  Serum IL-6 and CXCL-1 were measured at different time points (0, 60, and 90 minutes) after flagellin administration. Congenic animals homozygous for MOLF/Ei alleles at Tlr5 (B6.MOLF-Slc11a1 Ity3)exhibited the strongest response to flagellen stimulation out of all lines tested as indicated by significantly higher levels of serum IL-6 and CXCL-1. Parental strain MOLF/Ei exhibits the lowest serum IL-6 and CXCL-1 levels compared to parental C57BL/6Jand all 3 congenic lines. The results indicate the effect of Tlr5 is dependent on strain background. Therefore, it is unlikely Tlr5 is responsible for the Ity3 susceptible phenotype.