Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  18
• Reference
  J:105074 Darakhshan F, et al., Evidence for complex multigenic inheritance of radiation AML susceptibility in mice revealed using a surrogate phenotypic assay. Carcinogenesis. 2006 Feb;27(2):311-8
• ID
  MGI:3655635

Genes

Gene	Assay Type
Raml1	resistance/susceptibility
D18Mit146	PCR
D18Mit229	PCR
Rbbp8	reported elsewhere
Riok3	reported elsewhere
Rmc1	reported elsewhere
Npc1	reported elsewhere
Ankrd29	reported elsewhere
Lama3	reported elsewhere

Notes

• Experiment
  Susceptibility to radiation induced acute myeloid leukemia (AML) was mapped by genomic microsatellite analysis of 9 inbred strains. AML susceptible strains, such as CBA/H and CBA/Ca, exhibit a high frequency of chromosome 2 deletions in bone marrow cellsfollowing whole body irradiation whereas resistant strains, such as C57BL/6J, do not. Loci were detected on mouse Chromosomes 3, 4, 8, and 18. A limited (CBA/Ca x C57BL/6J)F1 x C57BL/6J backcross was used to confirm the QTLs.
  
  Raml1 (radiation induced acute myeloid leukemia 1) mapped to mouse Chromosome 18 with strongest linkage at D18Mit146 (4 cM) and D18Mit229 (4 cM). C57BL/6J-derived alleles at Raml1 confer sensitivity to radiation induced chromosome 2 deletions and, by association, AML. Loss of heterozygosity was not observed in this region. Potential candidate genes in this region are Rbbp8, Riok3 (3 cM), 3110002H16Rik (Mic1), Npc1 (4 cM), Ankrd29, and Lama3 (3 cM). Rbbp8 was identified as a strong candidate as this gene is upregulated in response toX-irradiation inCBA mice. Raml1 is syntenic to human 18q11, a region implicated in 322 cases of cancer.
  
  Raml2 (radiation induced acute myeloid leukemia 2) mapped to 61.8 cM on mouse Chromosome 3 near D3Mit319. This locus is syntenic to human Chromosome 4q25. Bomb1 (61.8cM) is a previously identified bone marrow lymphoma QTL co-localizing with Raml2.
  
  Raml3 (radiation induced acute myeloid leukemia 3) mapped to 55.2 cM on mouse Chromosome 4 near D4Mit248. This locus is syntenic to human Chromosome 1p34. Mycl1 (previously Lmyc; 55 cM) is a potential candidate gene for Raml3. Previously identified tumor susceptibility QTLs Thyls (55 cM) and Skts7 (56 cM) are found near Raml3.
  
  Raml4 (radiation induced acute myeloid leukemia 4) mapped to 33 cM on mouse Chromosome 8 near D8Mit178. This locus is syntenic to human Chromosome 19p13. Rab3a (32.6 cM) and Rab8a (33 cM) are potential candidate genes mapping near Raml4.
  
  Raml5 (radiation induced acute myeloid leukemia 5) mapped to mouse Chromosome 13 near D13Mit23. Nsd1, Mxd3 (formerly Mad3; 32 cM), and Msx2 (formerly Hox8; 32 cM) are potential candidate genes for Raml5.