Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:112375 Bouxsein ML, et al., Generation of a new congenic mouse strain to test the relationships among serum insulin-like growth factor I, bone mineral density, and skeletal morphology in vivo. J Bone Miner Res. 2002 Apr;17(4):570-9
• ID
  MGI:3689514

Genes

Gene	Assay Type
Igf1sl1	visible phenotype

Notes

• Experiment
  Igf1sl1 is previously identified QTL on mouse Chromosome 6 affecting levels of serum IGF-1. A congenic line named B6.C3-(D6Mit93-D6Mit150) carries a C3H/HeJ-derived interval encompassing Igf1sl1 between D6Mit93 (26.3 cM) to D6Mit150 (51 cM) on a C57BL/6J genetic background. Authors refer to this line as 6T, which is estimated to be approximately 99.8% C57BL/6J at the N9 generation. Congenic females display 11%-21% decreased serum IGF-1 at 6, 8, and 16 weeks of age, and congenic males display 13% decreased serum IGF-1 at 16weeks of age compared to C57BL/6J. Congenic osteoblasts also secreted 40% less IGF-1 compared to C57BL/6J osteoblasts. This study also examined the effects of serum IGF-1 on skeletal phenotypes.
  
  Congenic animals display decreased body fat percentage at12 weeks of age compared to C57BL/6J. Female congenic animals at 16 weeks of age display decreased femoral length, periosteal circumference, and total body areal bone mineral density (BMD) compared to C57BL/6J females. Female congenic animalsalso display significantly decreased bone volume fraction and bone thickness in the femur and vertebral bodies between 8-16 weeks of age. It appears that C3H/HeJ-derived alleles at Igf1sl1 affect bone development and acquisition, especially during times of rapid growth around 8-12 weeks of age.