Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:106111 Bernstein-Hanley I, et al., Genetic analysis of susceptibility to Chlamydia trachomatis in mouse. Genes Immun. 2006 Mar;7(2):1-8
• ID
  MGI:3692644

Genes

Gene	Assay Type
Ctrq1	resistance/susceptibility
D2Mit277	PCR amplified length variant

Notes

• Experiment
  Linkage analysis was performed on 169 animals from a (C57BL/6J x C3H/HeJ)F2 intercross to identify loci associated with susceptibility to Chlamydia trachomatis infection. Genome scan was conducted using 93 microsatellite markers at an average spacing of 16 cM. Parental strain C57BL/6J is more resistant to infection with C. trachomatis compared to parental strain C3H/HeJ. Animals were phenotyped by analyzing bacterial load in spleens after infection with C. trachomatis. In vitro analysis was also performed using INF-gamma-treated mouse embryonic fibroblasts (MEFs) derived from (C57BL/6J x C3H/HeJ)F2 animals infected with C. trachomatis.
  
  Ctrq1 (C. trachomatis resistance QTL 1) mapped to 69 cM on mouse Chromosome 2 near D2Mit277 (LRS=20.5). C3H/HeJ-derivedalleles at Ctrq1 confer susceptibility to C. trachomatis infection with a recessive mode of inheritance.
  
  Ctrq2 (C. trachomatis resistance QTL 2) mapped to 55 cM on mouse Chromosome 3 near D3Mit57 (LRS=19.9). The 1.5 LOD support interval spans 44.8 cM to66.2 cM from D3Mit49 to D3Mit350. C3H/HeJ-derived alleles at Ctrq2 confer resistance to C. trachomatis infection with dominant inheritance.
  
  Ctrq3 (C. trachomatis resistance QTL 3) mapped to 32 cM on mouse Chromosome 11 near D11Mit143 (LRS=19.5). C3H/HeJ-derived alleles at Ctrq3 confer susceptibility to C. trachomatis infection with dominant inheritance. This locus was reaffirmed by constructing a congenic line carrying the entire Ctrq3 region (D11Mit20 to D11Mit245) derived from susceptible strain C3H/HeJ on the genetic background of resistant strain C57BL/6J. B6.C3H-Ctrq3 congenics exhibit significantly greater bacterial load after C. trachomatis infection compared to C57BL/6J controls. IFN-gamma-treated MEFs derived from heterozygous B6.C3H-Ctrq3 animals infected with C. trachomatis also exhibit a greater level of bacterial replication compared to controls.