Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  11
• Reference
  J:117545 Farber CR, et al., Fine mapping reveals sex bias in quantitative trait Loci affecting growth, skeletal size and obesity-related traits on mouse chromosomes 2 and 11. Genetics. 2007 Jan;175(1):349-60
• ID
  MGI:3698552

Genes

Gene	Assay Type
D11Mit260	PCR amplified length variant
D11Mit255	PCR amplified length variant
Wg7	visible phenotype
D11Mit67	PCR amplified length variant
Kwq15	visible phenotype
D11Mit36	PCR amplified length variant
Brwq1	visible phenotype
Lwq9	visible phenotype
Tailq8	visible phenotype
D11Mit330	PCR amplified length variant
Feml4	visible phenotype
D11Mit280	PCR amplified length variant
Gfpq1	visible phenotype

Notes

• Experiment
  Wg2 (weight gain in high growth mice 2) and Wg4 (weight gain in high growth mice 4) were previously mapped to mouse Chromosomes 2 and 11, respectively. These loci were investigated further by construction of speed congenic lines carrying C57BL/6J-Socs2^hg-derived DNA at Wg2 or Wg4 on a CAST/EiJ genetic background. These lines are designated HG.CAST-(D2Mit329-D2Mit457) for the Wg2 congenic and HG.CAST-(D11Mit260-D11Mit255) for the Wg4 congenic. Parental strain C57BL/6J-Socs2^hg exhibits significantly increased growth trajectory after 3 weeks of age compared to CAST/EiJ. Over 700 F2 animals were produced from [C57BL/6J-Socs2^hg x HG.CAST-(D2Mit329-D2Mit457)]F2 and [C57BL/6J-Socs2^hg x HG.CAST-(D11Mit260-D11Mit255)]F2 intercrosses. Animals were analyzedat 9 weeks of age and screened for nineteen polymorphic markers on chromosome 2 (average resolution = 2.7 cM) and eleven markers on chromosome 11 (average resolution = 3 cM). Several novel body trait QTLs were identified and are described below.
  
  On mouseChromosome 11, linkage to 9-week body weight mapped to 18 cM near D11Mit67 (LOD=3.3). This locus explains 5.2% of the variance and is named Wg7. Male animals homozygous for CAST/EiJ-derived alleles at Wg7 display increased body weight at 9 weeks whereas female animals display decreased body weight, and the effect appears dominant. The Wg7 confidence interval spans 0 cM - 28.5 cM.
  
  Linkage to kidney weight, Kwq15, mapped to 11.5 cM (88 Mb) near D11Mit36 (LOD=8). This locus explains 12.4% of the variance and the confidence interval spans 7 cM - 17 cM. Homozygosity for CAST/EiJ-derived alleles at Kwq15 confers decreased kidney weight.
  
  Linkage to brain weight mapped to 18.5 cM (99 Mb) near D11Mit67 (LOD=6). This locus explains 9.5% of the variance and is named Brwq1. Homozygosity for CAST/EiJ-derived alleles confer decreased brain weight. The Brwq1 confidence interval spans 7 cM - 22.5 cM.
  
  Linkage to liver weight in males mapped to 10.5 cM (85 Mb) near D11Mit36 (LOD=7). This locus explains 11% of the variance and is named Lwq9. Homozygosity for CAST/EiJ-derived alleles at Lwq9 confer increased liver weight in male animals. The Lwq9 confidence interval spans 7 cM - 16 cM. Lwq9 overlaps with Kwq10 but non-concordance of respective allele effects suggest these are separate and distinct loci.
  
  Linkage to tail length, Tailq8, in males mapped to 19 cM (99 Mb) near D11Mit330 (LOD=8.5). CAST/EiJ-derived alleles at Tailq8 confer increased tail length in males with an additive mode of inheritance. This locus explains 13.1% of the variance. The Tailq8 confidence interval spans 10 cM - 34.5 cM.
  
  Linkage to femur length in females, Feml4, mapped to 5 cM (75 Mb) near D11Mit280 (LOD=16.5). This locus explains 24% of the variance. Homozygosity for CAST/EiJ-derived alleles at Feml4 confers decreased femur length in female mice. The Feml4 confidence interval spans 1.5 cM - 8.5 cM.
  
  Linkage to gonadal fat pad weight mapped to 32.5 cM near D11Mit255 (LOD=3.5). This locus explains 5.6% of the variance and is namedGfpq1. CAST/EiJ-derived alleles at Gfpq1 confer increased gonadal fat pad weight with a greater effect in females. The confidence interval spans 0 cM - 35 cM.