Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  17
• Reference
  J:117468 Kumar KG, et al., Quantitative trait loci for carbohydrate and total energy intake on mouse chromosome 17: congenic strain confirmation and candidate gene analyses (Glo1, Glp1r). Am J Physiol Regul Integr Comp Physiol. 2007 Jan;292(1):R207-16
• ID
  MGI:3699335

Genes

Gene	Assay Type
Kcal2	visible phenotype
Mnic1	visible phenotype
D17Mit19	PCR amplified length variant
D17Mit91	PCR amplified length variant
Glo1	reported elsewhere
Glp1r	reported elsewhere
Clps	reported elsewhere
Apom	reported elsewhere
Ppard	reported elsewhere

Notes

• Experiment
  Previously identified QTLs Mnic1 (macronutrient intake, carbohydrate 1) and Kcal2 (kilocalorie 2) at 20 cM on mouse Chromosome 17 were confirmed by analysis of speed congenic lines. B6.CAST- (D17Mit19-D17Mit91) carries a 38 cM region of CAST/EiJ-derived DNA from D17Mit19 (3 cM; 4.7 Mb) to D17Mit91 (37.8 cM; 63.5 Mb) on a C57BL/6J genetic background. The congenic interval contains Mnic1 and Kcal2. Donor strain CAST/EiJ displays significantly higher total daily caloric intake compared to background strain C57BL/6J. Likewise, congenic line B6.CAST-(D17Mit19-D17Mit91) displays 17% higher total calorie intake compared to C57BL/6J and prefers a carbohydrate diet (27% increase) over a fat diet.
  
  Glp1r at 18 cM is a positional candidate gene for Kcal2. Glp1r exhibits decreased expression in the hypothalamus and antral stomach, and increased expression in the pancreas, of CAST/EiJ mice compared to C57BL/6J mice. In B6.CAST- (D17Mit19-D17Mit91) mice, Glp1r expression is increased in the pancreas and decreased in the stomach compared to C57BL/6J mice. Sequence analysis of Glp1r revealed 3 polymorphisms between CAST/EiJ and C57BL/6J, one of which results in an amino acid substitution in exon 13 (C416Y). Glo1 at 16 cM is a positional candidate gene for Mnic1. Glo1 exhibits increased expression in liver and hypothalamus of congenic mice compared to C57BL/6J. Sequence analysis of Glo1 detected one silent polymorphism between CAST/EiJ and C57BL/6J. Other potential candidate genes mapping to the congenic interval include Clps (13.1 cM), Ppard (13.5 cM), and Apom.