Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:120148 Duarte N, et al., The Idd6.2 diabetes susceptibility region controls defective expression of the Lrmp gene in nonobese diabetic (NOD) mice. Immunogenetics. 2007 May;59(5):407-16
• ID
  MGI:3710089

Genes

Gene	Assay Type
Idd6.2	visible phenotype
D6Mit259	PCR amplified length variant
D6Mit304	PCR amplified length variant
Irag2	reported elsewhere

Notes

• Experiment
  Congenic line analysis was used to identify potential candidate genes for Idd6b at approximately 72 cM on mouse Chromosome 6. N.B-Idd6-15 is a congenic carrying C57BL/6-derived DNA from D6Mit200 (71.6 cM) to D6Mit304 (75 cM) on a NOD genetic background and excludes the Idd6b locus. A reverse congenic line, B6.NOD-Idd6, carrying NOD-derived DNA from D6Mit259 (67 cM) to D6Mit304 (75 cM) contains the Idd6b locus. Parental strain NOD is susceptible to type 1 diabetes compared to C57BL/6, with 89% of females and 42% of males affected by 40 weeks of age.
  
  Expression analysis of twelve genes within the Idd6b QTL interval was performed using RT-PCR on thymocyte RNA. Lrmp (71 cM) expression is significantly decreased in NOD thymocytes compared to C57BL/6 thymocytes. Furthermore, Lrmp expression segregates with the Idd6b locus as congenic B6.NOD-Idd6 displays decreased Lrmp expression relative to C57BL/6 controls. Congenic N.B-Idd6-15, which does not carry the Idd6b locus, displays similar levels of Lrmp expression as NOD controls. The Idd6b locus also appears to affect Lrmp expression in bone marrow and spleen cells from NOD, C57BL/6, and B6.NOD-Idd6 animals. Authors conclude that Lrmp is a plausible candidate gene for Idd6b but does not exclude other genes in the QTL region.