Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  2
• Reference
  J:86161 Ward JO, et al., Toward the genetics of mammalian reproduction: induction and mapping of gametogenesis mutants in mice. Biol Reprod. 2003 Nov;69(5):1615-25
• ID
  MGI:3714963

Genes

Gene
lsgd9a

Notes

• Experiment
  For ENU treatment of C57BL/6J males, a genome-wide set of microsatellite markers, polymorphic between B6 and C3H , was used to assay DNA from affected G3. For EMS treatment of 129 ES cells, a similar genome-wide set of microsatellite markers, polymorphic between B6 and 129, was used to assay DNA from affected G3 animals by PCR. Linkage was implicated by the association of phenotype with homozygosity for B6 (ENU) or 129 (EMS) alleles, respectively.If a higher degree of polymorphism was necessary, heterozygotes were bred to Mus castaneus mice, and F1 animals were intercrossed to generate F2 for further mapping.