Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  14
• Reference
  J:123129 De Franco M, et al., Slc11a1 (Nramp1) alleles interact with acute inflammation loci to modulate wound-healing traits in mice. Mamm Genome. 2007 Apr;18(4):263-9
• ID
  MGI:3717808

Genes

Gene	Assay Type
Ewrq1	visible phenotype
D14Mit194	PCR
Ang	reported elsewhere

Notes

• Experiment
  QTLs associated with ear wound healing were mapped using 317 (AIRmax x AIRmin)F2 animals. These lines were derived from 8 inbred strains and selected for maximal acute inflammatory reaction (AIRmax) or minimal acute inflammatory reaction (AIRmin). AIRmaxdisplays significant ear punch healing after 30 days whereas AIRmin does not. Linkage analysis was performed using 111 polymorphic markers.
  
  Significant linkage to ear wound healing mapped to 44.4 cM on mouse Chromosome 14 near D14Mit194 (LRS=12.8). This locus explains 21% of the variance and is named Ewrq1 (ear wound repair QTL 1). Ang at 18 cM is a potential candidate gene for Ewrq1. Musz3 (40.4 cM) and Heal12 (12 cM) are previously identified QTLs mapping to chromosome 14.
  
  Interaction of Ewrq1 with Salmonella resistance (RR) and susceptibility (SS) alleles at Slc11a1 was examined in AIRmax^RR, AIRmax^SS, AIRmin^RR, and AIRmin^SS sublines. Female AIRmax^SS animals display complete ear closure, and AIRmax^SS males display faster ear wound closure compared to AIRmax^RR males. This finding suggests Slc11a1^SS alleles help promote ear wound healing. Animals from AIRmin^SS and AIRmin^RR did not display ear wound closure.
  
  Suggestive linkage to wound healing mapped to 25.7 cM on mouse Chromosome1 near D1Mit236 (LRS=10.2). This locus explains 17% of the variance. Slc11a1 (39.2 cM) is a potential candidate gene for this locus. Previously identified wound healing QTL Stheal1 (49.2 cM) also maps to chromosome 1.