Mapping Data

Experiment

• Experiment
  TEXT-Meta Analysis
• Chromosome
  1
• Reference
  J:123776 Wergedal JE, et al., Mapping genetic loci that regulate lipid levels in a NZB/B1NJxRF/J intercross and a combined intercross involving NZB/B1NJ, RF/J, MRL/MpJ, and SJL/J mouse strains. J Lipid Res. 2007 Aug;48(8):1724-34
• ID
  MGI:3720118

Genes

Gene
Chldq10
Tglq1

Notes

• Experiment
  Linkage analysis was performed on 542 female (NZB/BlNJ x RF/J)F2 intercross animals to identify QTLs associated with serum lipid levels. F2 female animals were fed a CHOW diet and sacrificed at 10 weeks of age for phenotype analysis. A panel of 94 polymorphic markers was used for linkage analysis. Parental strain NZB/BlNJ displays 48% increased plasma cholesterol and 57% increased plasma HDL compared to RF/J, and parental strain RF/J has 58% increased triglycerides and 10% higher body weight compared to NZB/BlNJ.
  
  Data from a previously studied population of 621 (MRL/MpJ x SJL/J)F2 females was also used for a combined analysis. A set of 231 microsatellite markers was used to genotype the combined data set.
  
  In the combined cross analysis [Table. 2.]:
  
  A QTL on Chromosme 1, Chldq10 (cholesterol and HDL QTL 10), was detected in the combined data set with LOD=22.87 for plasma cholesterol and LOD=23.08 for plasma HDL at 96 cM.
  
  A triglyceride QTL, Tglq1 that was originally mapped in J:106033 in 2006 using an(MRL/MpJ x SJL/J)F2 intercross was confirmed in the combined data analysis. In the original cross it mapped to 76.2 cM on Chr 1 with a LOD=3.80. It did not map with significance here in the (NZB/BlNJ x RF/J)F2 intercross but peaked at 76.0 cM with a LOD=5.06 when the data from both crosses was combined. This QTL was specific to the (MRL/MpJ x SJL/J) F2 cross.