Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  15
• Reference
  J:124159 Devor M, et al., Sex-specific variability and a 'cage effect' independently mask a neuropathic pain quantitative trait locus detected in a whole genome scan. Eur J Neurosci. 2007 Aug;26(3):681-8
• ID
  MGI:3721670

Genes

Gene	Assay Type
Pain2	visible phenotype
D15Mit277	PCR amplified length variant

Notes

• Experiment
  Linkage analysis was performed on 370 (C3H/HeN x C58/J)F1 x C3H/HeN backcross animals to identify genetic loci involved with neuropathic pain. Animals were surgically denervated at the hindpaw at 2-3 months of age and evaluated weekly for autotomy.
  
  One-third of the animals were group-housed and two-thirds of the animals were individually-housed. Parental strain C3H/HeN typically displays high pain (autotomy) phenotype and parental strain C58/J typically displays low pain phenotype. Investigators observed group housing affects pain response phenotype. A panel of 100 polymorphic markers spaced 15 cM - 20 cM apart was used for genome scan.
  
  A locus on mouse Chromosome 15, the previously identified Pain1 QTL at 44 cM, was detected only in the individually-housed backcross mouse population with a higher level of significance in females. Peak linkage to autotomy in females mapped to 32 cM at marker D15Mit277 with LOD=3.3. Pain1 explains 17.1% of the variance in females and 3.4% of the variance in males.
  
  10.29.2015 Curator Note: Because Pain1 was originally mapped in J:70963 in 2001 using (AXB) and (BXA) RI strains, which differ from the mouse population used here, we consider the current study a separate mapping experiment and have equated this QTL with Pain2. Pain2 was originally mapped in J:99476 in 2005 using the same backcross population used here.
  
  Homozygosity for C3H/HeN derived alleles at Pain2 confers high autotomy in females with reduced penetrance (40%). However, heterozygosity for C58/J and C3H/HeN derived alleles at Pain2 confers low autotomy in female animals with high penetrance (97%).