Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:82643 Boulton E, et al., Low-penetrance genetic susceptibility and resistance loci implicated in the relative risk for radiation-induced acute myeloid leukemia in mice. Blood. 2003 Mar 15;101(6):2349-54
• ID
  MGI:3722395

Genes

Gene	Assay Type
Ramls2	resistance/susceptibility
D6Mit384	PCR

Notes

• Experiment
  The inbred strain CBA/H displays increased susceptibility to radiation-induced acute myeloid leukemia (lifetime incidence of 20%) compared to inbred strain C57BL/6 (lifetime incidence of 7%). Linkage analysis was performed on a population of (CBA/H x C57BL/6)F1 x CBA/H animals to identify QTLs associated with susceptibility to radiation-induced acute myeloid leukemia (r-AML). A total of 1087 backcross animals were exposed to 3 Gy x-rays resulting in 67 animals affected with r-AML. A panel of 138 polymorphic markers spaced approximately 22 cM apart was genotyped in the 67 affected backcross animals. Two low-penetrance QTLs named Ramls1 (radiation-induced acute myeloid leukemia sensitivity 1) and Ramls2 (radiation-induced acute myeloid leukemia sensitivity2) were identified. It is thought Ramls1 and Ramls2 are involved in separate leukemogenic processes.
  
  A minor locus for r-AML sensitivity mapped to 100 cM on mouse Chromosome 1 near D1Mit150. This locus is named Ramls1 (radiation-induced acute myeloid leukemia sensitivity 1). Excess homozygosity for CBA/H-derived alleles at Ramls1 is associated with increased relative risk (RR) for r-AML. A previously identified stem cell frequency QTL named Scfr1 (98 cM) maps near this locus.
  
  A minor locus for r-AML sensitivity mapped to 27 cM on mouse Chromosome 6 near D6Mit384. This locus is associated with r-AML resistance and is named Ramls2 (radiation-induced acute myeloid leukemia sensitivity 2). Excess heterozygosity for CBA/H and C57BL/6 alleles at Ramls2 is associated with increased r-AML RR. Ramls2 is also associated with a high frequency of chromosome aberrations (maker chromosomes, trisomy, monosomy) in r-AML animals.
  
  Suggestive linkages to r-AML sensitivity mapped to 7 cM on mouse Chromosome 4 near D4Mit292 (P=0.021) and 34 cM on mouse Chromosome 13 near D13Mit248 (P=0.043).