Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  11
• Reference
  J:126552 Sancho-Shimizu V, et al., Molecular genetic analysis of two Loci (ity2 and ity3) involved in the host response to infection with salmonella typhimurium using congenic mice and expression profiling. Genetics. 2007 Oct;177(2):1125-39
• ID
  MGI:3762329

Genes

Gene	Assay Type
Ity2	resistance/susceptibility
D11Mit112	PCR
Mpo	PCR
Nos2	PCR
Ity2a	resistance/susceptibility
D11Mit109	PCR
D11Mit26	PCR
Havcr2	reported elsewhere
Ity2b	resistance/susceptibility
D11Mit5	PCR
D11Mit8	PCR

Notes

• Experiment
  Previously identified Salmonella susceptibility QTLs Ity2 and Ity3 were confirmed and refined by linkage analysis of 232 (C57BL/6J x MOLF/EiJ)F2 animals. Parental strain MOLF/EiJ is susceptible to Salmonella typhimurium infection compared to parental strain C57BL/6J. Ity2 and Ity3 were further analyzed using subcongenic lines and candidate genes were identified using gene expression profiling after infection with S. typhimurium.
  
  Ity2 was refined to an interval on mouse Chromosome 11 between D11Mit112 (32 cM) and Mpo (49 cM). Peak linkage occurred at Nos2 (45.6 cM) with LOD=7.8. Ity/Ity2 congenic animals were constructed by selecting for MOLF/EiJ-derived donor DNA at D1Mcg4 (Ity) and D11Mit84 - Mpo (Ity2) on a C57BL/6J genetic background. This line was then bred into two different subcongenic lines, RecD and RecI. At least two different loci were found to contribute to S. typhimurium resistance and were named Ity2a and Ity2b. Ity2a maps to a 23 Mb interval from D11Mit109 (19 cM) to D11Mit26 (33.3 cM) while Ity2b maps to a 13 Mb interval from D11Mit5 (37 cM) to D11Mit8 (46 cM). Gene expression analysis identified Havcr2 as a promising candidate gene for Ity2a. No candidate genes were identified for Ity2b.
  
  Ity3 was refined to an interval on mouseChromosome 1 between D1Mit135 (59.7 cM) and D1Mit201 (79 cM) with peak linkage of LOD=4.1 occurring between D1Mit218 (67 cM) and D1Mit100 (71.5 cM). Ity/Ity3 congenic animals were constructed by selecting for MOLF/EiJ-derived donor DNA at D1Mcg4 (Ity) and D1Mit135 - D1Mit17 (Ity3) on a C57BL/6J genetic background. This line displayed significantly reduced survival time after S. typhimurium infection. Subcongenic lines RecA, RecB, RecC, and RecE were constructed and localized the Ity3 critical region to a34 Mb interval between D1Mit193 (68 cM) and D1Mit63 (87.8 cM). Gene expression analysis identified Lax1 and Chi3l1 as potential candidate genes for Ity3.