Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  9
• Reference
  J:127710 Yu H, et al., Mouse chromosome 9 quantitative trait loci for soft tissue regeneration: Congenic analysis and fine mapping. Wound Repair Regen. 2007 Nov-Dec;15(6):922-7
• ID
  MGI:3766478

Genes

Gene	Assay Type
Stheal8	visible phenotype
Stheal9	visible phenotype
Tbx18	reported elsewhere
D9Mit196	PCR amplified length variant
D9Mit9	PCR amplified length variant

Notes

• Experiment
  The effects of tissue regeneration QTLs Stheal8 (33 cM) and Stheal9 (43 cM) on mouse Chromosome 9 were further analyzed using congenic animals. A congenic line was constructed which carries MRL/MpJ-derived DNA from D9Mit207 (33 cM) to D9Mit151 (72 cM) onan SJL/J genetic background. This donor segment contains both Stheal8 and Stheal9. Donor strain MRL/MpJ displays significantly better wound healing compared to background strain SJL/J.
  
  SJL.MRL-(D9Mit207-D9Mit151) male congenic displayed increased healing with 12% smaller ear wound holes at 15- and 25-days after ear punch compared to SJL/J control males. This trend was also seen in female congenic animals although not at a statistically significant level.
  
  Fine map analysis of the chromosome 9 region containing Stheal8 and Stheal9 was performed using an extended set of 1,564 male and female (MRL/MpJ x SJL/J)F2 animals and 23 polymorphic markers spaced approximately 4.2 Mb apart.
  
  Four separate peaks were detecting suggesting Stheal8 and Stheal9 may be comprised of multiple linked loci.
  
  The Stheal8 peak mapped between 61 Mb - 64 Mb with LOD=4.3 in males and LOD=5.3 in females. A separate peak distal to Stheal8 was detected at 69 cM - 85 Mb. Adam10 and Ttk are potential candidate genes for this locus.
  
  Stheal9 was the third peak at 85 Mb (D9Mit196) - 90 Mb (D9Mit9) with LOD=8.8 in males and LOD=7.7 in females. Stheal9 displayed the strongest effect explaining 36% of the phenotypic variance. Tbx18 is a potential candidate gene for Stheal9.
  
  The fourth peak mapped distal to Stheal9 at 94 Mb - 97.5 Mb.
  
  Bayesian shrinkage analysis reduced the Stheal8 interval to 1.7 Mb between 60.8 Mb and 62.5 Mb. Stheal9 was reduced to a 1.3 Mb interval between 86.3 Mb and 87.6 Mb, and peak 4 was reduced to a 1.3 Mb interval between 94.2 Mb and 95.5 Mb.