Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  1
• Reference
  J:137521 Jordan MA, et al., Slamf1, the NKT cell control gene Nkt1. J Immunol. 2007 Feb 1;178(3):1618-27
• ID
  MGI:3818442

Genes

Gene	Assay Type
Nktcn1	visible phenotype
Slamf1
Slamf6

Notes

• Experiment
  Microarray analysis was performed on thymus RNA from 4 week old NOD.Nkrp1^b.Nkt1^b congenic animals and NOD.Nkrp1^b congenic animals to screen candidate genes for Nktcn1. The NOD.Nkrp1^b congenic carries C57BL/6J-derived DNA from D6Mit105 (45.5 cM) toD6Mit135 (62.3 cM) on a NOD genetic background. The NOD.Nkrp^b.Nkt1^b congenic carries C57BL/6J-derived DNA from D1Mit369 (73 cM) to D1Mit155 (telomere) on a NOD genetic background in addition to the NOD-derived chromosome 6 interval. The formal QTL designation for Nkt1 is Nktcn1. Nkrp1 is synonymous with Klrb1c. Previously identified lupus susceptibility locus Sle1b (89.5 cM) maps near Nktcn1.
  
  Twenty-one genes displaying expression differences between NOD and C57BL/6J mapped to the Nktcn1 region, 15of which are located in the 95% confidence interval. The most promising candidate genes for controlling NKT cell number are Slamf1 (93.3 cM) and Slamf6 (89.5 cM). Gene expression of Slamf1 and Slamf6 was examined in thymic and splenic lymphocytes from congenic animals. It was observed that NOD.Nkrp^b.Nkt1^b animals expressed significantly higher amounts of SLAM proteins on cell sufaces compared to NOD.Nkt1^b parentals. Authors hypothesize control of NKT cell numbers may be mediated by differentialexpression of Slamf1 with additionial influence by Slamf6.